The Isle of Wight Birth Cohort

The Birth Cohort is a single centre study designed to represent the community population. All children born on the Isle of Wight in a defined period were eligible for inclusion. Ethics approval was obtained from the local/National Ethics Committees at recruitment of the birth cohort between January 1989 and February 1990, and subsequently at each assessment. The cohort was recruited through the 1509 women who gave birth to 1536 children on the IOW during the recruitment period. All 1509 mothers were recruited and consented to complete questionnaires and provide samples soon after birth. Parental consent was obtained from 1456 of the 1536 children for inclusion into a longitudinal study of asthma and allergic disease.

The Isle of Wight Birth Cohort

The children in the Birth Cohort have been seen on 6 occasions over the course of 26 years, at 1, 2, 4, 10, 18 and 26 years (Table 1). Extremely high retention rates have been obtained at all-time points. Cohort participants who attended or did not attend at various assessments were compared for information collected at birth when parents of all 1536 infants responded and provided basic information on family history of allergy, birth weight, social status and exposures to pets and smoking (Table 2).

The Isle of Wight Birth Cohort

Hospital records were used to gather information on maternal height and weight at week 14 (±4) of gestation, pregnancy characteristics and complications, and birth characteristics. A sample of maternal blood and cord blood at birth and children’s blood from a heel prick was collected at 7 days of age on Guthrie cards.

Questionnaires, both study specific and standardised questionnaires (International Study of Asthma and Allergic Diseases in Childhood from 10 years onwards when these became available) seeking information on asthma and allergy status and common environmental exposures were completed by most participants or their parents at various assessments throughout childhood and early adult life. Mothers completed a questionnaire soon after the birth of her child , and at one and two years, children were seen by a doctor, nurse or health visitor and a questionnaire completed. If parents reported any allergy related symptoms in their child, they were asked to attend the clinic for a visit where examination and allergy skin prick tests (SPT) were carried out. Physical examination at all assessments included height, weight, and signs of allergic diseases such as wheeze and eczema. At 4, 10, 18 and 26 years, all participants were invited to attend the research centre for an assessment, which included questionnaire, physical examination, and skin prick test. At 10, 18 and 26 years, spirometry and bronchial provocation tests were carried out and blood and urine samples were collected. Exhaled nitric oxide was measured at 18 and 26 years. A subgroup of children were invited for sputum induction at 10 and 18 years. At all ages, those who could not attend the centre for a personal visit were asked to complete a telephone or postal questionnaire. At 26 years, on-line questionnaires were first introduced, in addition to telephone and postal questionnaires, to achieve optimal participation. Where possible, participants were asked to give permission for access to their medical records, which provided more accurate data regarding physician diagnosis and treatments given.

Serum total IgE was measured at birth (in the cord serum), and again at 10 and 18 years. Specific IgE screens for aero and food allergens were carried out at 10 and 18 years. Serum leptin and urinary cotinine were measured at 10 and 18 years. In a subgroup, a panel of cytokines was measured at 10 and 18 years. Genome-wide genotyping is being carried out currently and data will be available shortly. Genome-wide epigenotyping with DNA methylation was carried out in a subgroup of participants in whole-blood derived DNA collected at 18 years  and is now being extended to all participants with blood samples available at birth (using Guthrie cards), and 10 and 18 years.

The Isle of Wight Birth Cohort

What has it found?

Nearly 100 original articles have been published, describing prevalence, natural history and genetic and environmental risk factors for asthma and allergic diseases up to 18 years of age, while data collected at the age of 26 years are currently being analysed. A list of publications arising from the IOW Birth Cohort can be found at https://www.ncbi.nlm.nih.gov/pubmed/?term=Isle+of+Wight+Birth+Cohort.

Prevalence

We described the population prevalence of allergic disorders at various ages in this unselected birth cohort. The prevalence was generally described as period prevalence, i.e. in the last 12 months at each assessment. We used study-specific questionnaires in the first 3 assessments and later repeated these questionnaires as well as using standardised International Study of Asthma and Allergy in Childhood (ISAAC) questionnaires¹, which had become available between the 4 and 10 year follow-up. The overall prevalence of one or more allergic disease varied from ~25% in the first 2 years to 40% at 4 and 50% by the age of 18 years.^2-6

Asthma. Reported asthma at 1, 2 and 4 years, defined as recurrent wheezing, increased from 8.7% at 1 years to 14.9% at 4 years.^2,4,7 At age 10, 18 and 26 years, we characterised cohort children extensively for asthma and allergic diseases using standardized questionnaires, lung function, bronchial provocation tests, and sputum induction.^5,6,8-11 We also described wheezing phenotypes during the first 10 years of life, identifying that more severe disease had an early onset and could be distinguished from more transient disease using risk scoring systems .^8,9,12,13 We investigated early life risk factors for the development of asthma and bronchial hyper responsiveness (BHR) during later childhood^10,13 and how these factors influence symptom expression in those with BHR¹⁰ and induce earlier onset of disease.¹⁴

Nearly 5% of adolescents reported wheezing in the absence of diagnosed asthma and showed few pathophysiological hallmarks of asthma. This ‘undiagnosed wheeze’ phenotype was associated with smoking and paracetamol use.¹⁵ Applying cluster analysis methods on IOW Birth Cohort data, we have defined wheeze and rhinitis clusters to explore phenotypes of these conditions using adolescents.^16,17 By 18-years severe asthma clusters with evidence of impaired lung function, high morbidity and higher smoking prevalence were identifiable.

Allergic rhinitis. Rhinitis was defined as nasal and/or eye symptoms of sneezing, rhinorrhoea, nasal blockage, and streaming/itchy eyes when not having a “cold” or respiratory infection. At 1 and 2 years, the prevalence was low (~3%) but gradually increased so by 26 years it had reached 42%.^{2-4,7,16,18-20}

Atopic dermatitis. Atopic dermatitis, using modified Hannifin and Rajka definition was approximately 10% during early childhood.^2-4,7,21

Peanut allergy. We were among the first to describe the prevalence of peanut allergy in 4 year old children in the IOW Birth Cohort.²² More recently we described the natural history of peanut allergy over the first 18 years of life.²³ Subsequently, the prevalence rates in the IOWBC was compared with another cohort of children of the same age, born a few years later on the IOW and assessed for peanut allergy. This showed that sensitisation increased 3-fold and clinical allergy to peanut doubled during the 1990s.²⁴ In early 2000, we recruited another birth cohort on the IOW (Food Allergy and Intolerance Research cohort) and assessed children for food allergy during early childhood. Therefore, we were able to compare prevalence in these 3 sequential cohorts of children, all aged 3-4 years but born 5-6 years apart. We found that after the initial rise in 1990s, the peanut prevalence in the UK stabilized during the last decade.²⁵

Allergic sensitisation. The relationship of allergic sensitisation, asthma, and allergic disease was investigated from the ages of 4 to 26 years.^4,12,26-28 Atopy (SPT positive to any allergen) was 29% at 4 years.⁴ A strong relationship was found between allergic diseases such as asthma with house dust mite, allergic rhinitis with grass pollen and eczema with egg.⁷ Various childhood atopic phenotypes were described and their relationships with wheeze and asthma were defined. The population-attributable risk of atopy for asthma was 44%, for rhinitis it was 46% and for eczema, 32%.²

We recently showed that fractional exhaled nitric oxide (FeNO) is associated with atopy and atopic asthma, but not with non-atopic asthma. This has implications in the use of FeNO for the diagnosis and management of asthma.²⁷

Natural history

Overall, the prevalence of wheeze and asthma has continued to rise from early childhood to early adult life. However, there was fluidity such that a proportion of children who had wheeze at one follow-up were not wheezing at the next, but other non-wheezing children had acquired wheeze so that the trend of period prevalence remained upwards. The remission, relapse, and new onset (in those who were previously disease free) was also seen in other allergic manifestations including eczema, rhinitis, food allergy and allergic sensitisation.^20,23,29-32 However, the net trend for asthma and rhinitis was generally upwards, for eczema and food allergy there was a relatively high prevalence in early childhood followed by overall stable figures of around 10-15% for eczema and 1-3% for food allergy.

We were among the first to report that children with egg allergy in infancy have a 5 to 6-fold increased risk of acquiring aeroallergen sensitisation and respiratory symptoms by age 4,³³ thus shifting the allergic phenotype from food allergy to aeroallergen sensitisation with associated asthma and rhinitis.

Risk factors for allergic diseases

Sex: Boys suffered from asthma, eczema, rhinitis and atopy (allergic sensitisation) more than girls throughout childhood and early adult life (up to age 26 years). For asthma, a gender reversal occurs during adolescence; thus at age 18 girls had more asthma than boys.^{2,4,6-8,20,21,31,34}

Parental allergy. As expected, parental asthma and allergy had a consistent effect on childhood asthma and eczema over the entire childhood and adolescent period^{2,3,6,7,18,21,34-36} with some disease specificity such that parental asthma increased the risk of asthma more than eczema or rhinitis .⁴ We also showed that the risk is sex specific, such that boys had higher risk of asthma when their fathers were affected by asthma, while girls had a higher risk when mothers were diagnosed with asthma.³⁵

Breastfeeding and asthma. The effect of breastfeeding on asthma remains controversial. We showed that breast feeding for at least 3 months protects against early childhood wheezing, ^3,7 possibly as a result of attenuating the adverse effect of respiratory infections and maternal smoking on asthma.^37-39 We also showed that breastfeeding is associated with better lung function at 10 and 18 years of age.^38,40 However, the effect on allergic diseases in later childhood and adolescence was less clear.⁴¹ Our data suggest that some of the conflicting results on method of feeding and allergy may be due to reverse causation.⁴²

Low birth weight: Low birth weight was shown to be a risk factor for asthma, atopy and lung function.^{34,36,39,43-45}

Exposure to smoking: We demonstrated the adverse effects of maternal smoking exposure on the developing foetus with increased risk of wheeze and nasal symptoms during infancy,^2,7,18,34,46 and of active smoking on lung health during adolescence.^6,15 Maternal smoking also had an effect on eczema at age 4 years.⁴⁶ The genetic susceptibility and epigenetic mechanisms mediating this susceptibility have been studied (see below). These findings have paved the way to identify susceptible smokers at risk of future COPD.

Lower socio-economic group: Children among the lower socioeconomic group had a higher level of infant wheezing even after adjusting for confounding factors such as lack of breast feeding and maternal smoking. ^2,3,7

Presence of pets: We have not found an effect of exposure to furry pets on asthma or other allergic diseases at any age.^{2,3,18,21,34,47} A similar conclusion was reached in a meta-analysis of data from various European birth cohorts including the Isle of Wight.⁴⁸

Season of birth: We have found effect of season of birth with a higher level of asthma and rhinitis during the summer.^2,3,7,49 Autumn births were associated with rhinitis and autumn and winter combined had more eczema.

Cord and maternal IgE: The presence of cord IgE was associated with maternal IgE³⁶ and increased the risk of allergic sensitisation at 4 years.³ We also showed that IgE at birth (cord IgE) decreases with increasing birth order.⁵⁰ This provides an alternative explanation to the hygiene hypothesis for the lower incidence of allergic disease observed in younger siblings, as it seems they are born with lower cord IgE. We subsequently showed that this effect on children may be transmitted from the mother as their IgE also decreases with the increasing number of children they delivered.⁵¹ We demonstrated that the birth order effect is dependent on genetic susceptibility. An interaction between an IL13 gene polymorphism (rs20541) and birth order was found, whereby the effect of this SNP on skin test (ages 4 to 18), total IgE (age 10), and inhalant screen (age 10) was restricted to first-born children.⁵²

Predictors

Using longitudinal and repeated assessments of allergic disease in our birth cohort and available information on risk factors and biomarkers, we attempted to identify predictive markers for asthma and allergy.^13,33,53,54 We initially focused on cord blood IgE and found that an elevated cord IgE increases the risk of allergic sensitisation during childhood.^53,55 Although it did not increase the risk of respiratory symptoms in early childhood, it did increase the risk of asthma at age 10.⁵⁵ However, the sensitivity of cord IgE was too low to be used as a predictive marker for allergic disease.^53,54 Another important issue in paediatric allergy is the outcome of infant wheeze and its relationship with later childhood asthma. We developed predictive scores, based on a set of 4 risk factors (maternal asthma, allergic sensitisation, recurrent chest infections, and absence of nasal symptoms). Among children with a risk score of 4, 83% persisted with their wheeze, while those with a risk score of zero, 80% went into remission.¹³ Egg allergy combined with eczema during infancy had a high (>80%) positive predictive value for allergic sensitisation and respiratory symptoms.³³

Genetics

We identified a novel gene (ATPAF1) association with childhood asthma using a genome-wide approach on pooled DNA.⁵⁶ Cohort data were also utilized to identify a novel gene regulating neutrophil function, which is responsible for severity in cystic fibrosis.⁵⁷ Using a candidate gene approach, we investigated the association of IL13 with cord IgE and atopic eczema.^58,59 We have also demonstrated that filaggrin loss of function mutations contribute to allergic comorbidity including food allergy.^60-63 Gene-gene interaction between GATA3 and STAT6 with IL13 on rhinitis and eczema, respectively, was demonstrated.^64,65

Exposure to tobacco smoke increases the risk of wheeze and lung function deficit. We have shown the interaction of pre- and postnatal smoking exposure with genetic polymorphisms in genes encoding IL-13, IL-1R antagonist and GSTM2-5 on development of asthma and lung function.^66-68 Exposures related to birth order modified the effect of IL13 polymorphism on allergic sensitisation while filaggrin loss of function mutations modified the effect of breast-feeding on eczema.^52,69

Epigenetics

We have explored epigenetic mechanisms using genome-wide DNA methylation.^49,70-82 Common environmental exposures such as smoking alter epigenetic profile, which in turn is shown to be associated with the risk of allergic diseases (Table 5). Interestingly, tetanus vaccination between 10 and 18 years was related to differential methylation, which in turn reduced the risk of asthma at 18 years.⁷⁹ Using a two-stage model we showed that genetic variants in combination with living conditions, for instance use of oral contraceptive in girls, may change the DNA-methylation, which in turn modifies the genetic associations related to asthma.⁸² The interaction of DNA methylation with genetic variants was demonstrated for a number of allergic markers and diseases.^70-73,77

The Isle of Wight Birth Cohort

Strengths: The IOW Birth Cohort is an unselected whole population cohort that truly represents the community from which the cohort is drawn. There has been a high retention rate of over 70% throughout, with availability of information and samples from the parental generation, comprehensive assessment that covered not only asthma but all chronic allergic conditions, prospective and extensive phenotyping, as well as genome-wide (epi)genotyping.

Weaknesses: Despite reletively high retention, some self-selection was observed in chidren who attended at various assessments. For instance, at 18 and 26 years, girls attended more than boys and children who were assessed tended to have a lower proportion of parental smoking and low (<2.5kg) birth weight. However, as the follow-up rates were consistently high (80-90%) and imbalances were few, this reletively modest selection bias does not affect the validity or generalisability of the findings. The Isle of Wight is a relatively small island (~20 miles across) and therefore there is a lack of diversity, both in terms of environment (no industrial exposure) and race (>90% Caucasian), hence raising potential questions regarding generalisability of findings. The population is, however, not genetically inbred and there is frequent movement of people from mainland England.

The Isle of Wight Birth Cohort

We encourage collaboration to maximise the use of data and samples. We are in the process of finalising details of how the data can be accessed and the process of submitting an application to access the data. Please contact Mr Stephen Potter.

The tables below will give details about what data is available.

A study of Coronavirus infection in the Isle of Wight Whole Population Birth Cohort

You may remember that you are part of the world famous Isle of Wight Birth Cohort. More than 70% of you participated in the last assessment at the age of 26 years. This study has already contributed enormously to the knowledge of asthma and allergy, with more than 100 scientific papers, and has helped clinicians all over the world in better managing asthma and allergic diseases.

Now the world is struggling with a new threat that has impacted all our lives and caused huge suffering worldwide. Coronavirus infection is generally mild in children and young adults and they recover quickly. Indeed, many would have so mild an infection that they do not know about it or would have regarded the symptoms as due to a mild cold or flu. Nonetheless, the short- and long-term effects of this mild or asymptomatic infection in young adults are not yet known. We are planning to study these effects in the cohort participants. Therefore, we are asking you to let us know if you developed symptoms suggestive of Coronavirus, and the NHS helpline advised you to isolate yourself, or if you had a positive test for Coronavirus. We would like to gain a bit more information on the symptoms and duration of your illness and take a blood sample to look for the effect of Coronavirus on your immune system.

We would really welcome your involvement!

Please take the time to read the Information Sheet and then visit the website below to complete a short online questionnaire:

• Participant Information Sheet: Click Here to Open
• Questionnaire: www.isurvey.soton.ac.uk/38062

Best Wishes

Prof. S. Hasan Arshad and the Isle of Wight Birth Cohort Study Team